Table 1 Primers sequence of target genes
From: The anti-inflammatory effect of kaempferol on early atherosclerosis in high cholesterol fed rabbits
Target genes | Gene bank accession no. | Primers | Annealing temperature (°C) | Size (bp) |
|---|---|---|---|---|
β-Actin | NM_001101683.1 | 5′ -TTCCAGCCCTCCTTCCT- 3′ | 56 | 315 |
5′ -GCCCGACTCGTCATACT- 3′ | ||||
E-sel | NM_001082312.1 | 5′ - AATGGCAGATACAGAGAACT- 3′ | 49 | 219 |
5′ -TGGCTTGGAAGAGAATAACT- 3′ | ||||
ICAM-1 | AB128157.1 | 5′ -GACATTCTTGAACAGTGACAG- 3′ | 46 | 183 |
5′ -CGGACACAGCTCTCAGTA- 3′ | ||||
VCAM-1 | NM_001082152.1 | 5′ -GGAGACACTGTCATTATCTCCTG- 3′ | 58 | 336 |
5′ -TCCTTTCATGTTGGCTTTTCTTGC- 3′ | ||||
MCP-1 | M28883.1 | 5′ -GGTGTAAAGGCAGGTGTG- 3′ | 52 | 214 |
5′ -AGGATAGGAAAGGATGGG- 3′ |