Figure 2
From: Critical role of leukotriene B4 receptor signaling in mouse 3T3-L1 preadipocyte differentiation
Effects of LOX inhibitors and the BLT antagonist on mouse 3T3-L1 preadipocyte differentiation. (A), (B) and (C): Effects of NDGA ( LOX inhibitor, A), AA-861 ( 5-LOX inhibitor, B), or ONO-4057 ( a specific BLT antagonist, C) on lipid accumulation in mouse 3T3-L1 preadipocytes. Mouse 3T3-L1 preadipocytes were treated with NDGA, AA-861 or ONO-4057 for 6 days. Then, accumulation of triacylglycerol (TG), a marker of lipid accumulation (bottom panel), in matured adipocytes was measured and expressed as TG contents (μg/mg protein). Each column represents the mean ± SEM from 4-8 independent experiments. *P<0.05, **P<0.01 vs. vehicle control. Upper left panel shows representative photographs of differentiated mouse 3T3-L1 preadipocytes treated with vehicle for 6 days. Upper right panel shows representative photographs of undifferentiated mouse 3T3-L1 preadipocytes treated with NDGA, AA-861 or ONO-4057 for 6 days. Cells were stained with Oil Red O method to visualize lipid accumulation. Scale bar represents 100 μm.