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Figure 2 | Lipids in Health and Disease

Figure 2

From: Modulating the inflammatory properties of activated microglia with Docosahexaenoic acid and Aspirin

Figure 2

50,000 EOC20 Microglia were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μM DHA for 24 hours. After 24 hours, cells were stimulated with TLR agonists. At 24 hours post TLR stimulation, supernatants and cellular extracts were collected; total incubation time was 48 hours. Cellular extracts were assayed for total Glutathione content. Values were normalized to the untreated. Untrtd = Untreated; Imi = Imiquimod (10μg/mL); PIC = Poly I:C (1μg/mL); DHA = Docosahexaenoic Acid (50μM). *,# = p< 0.05; ** = p<0.005, ### = p<0.0005.

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