Effect of n-3 and n-6 unsaturated fatty acids on prostate cancer (PC-3) and prostate epithelial (RWPE-1) cells in vitro

Table 5 Changes seen in the survival, FR generated, and LXA ₄ , TNF-α and IL-6 secreted by PC-3 cells when supplemented with different doses of fatty acids for 48 hours

Treatment	PC-3% survival	FR generated% of control	LXA4 levels% of control	IL-6% of control	TNF-α% of control
Control	100 ± 1.86	100 ± 2.09	100 ± 2.60	100 ± 0.07	100 ± 6.34
LA 50 μM	105.00 ± 1.81*	104.31 ± 6.33*	56.00 ± 6.61***	67.06 ± 6.11**	53.75 ± 6.67***
LA 150 μM	95.00 ± 4.62*	102.65 ± 6.77*	72.00 ± 4.00**	71.18 ± 4.58**	71.25 ± 3.97**
GLA 25 μM	88.61 ± 4.39*	68.49 ± 3.35**	187.49 ± 2.11***	158.71 ± 9.66***	137.00 ± 3.94**
GLA 50 μM	82.53 ± 3.72**	74.67 ± 2.34**	179.34 ± 0.79***	148.57 ± 8.14***	148.81 ± 4.64***
GLA 75 μM	67.74 ± 3.81***	58.97 ± 5.11***	200.20 ± 2.94***	174.32 ± 7.32***	259.95 ± 6.95***
AA 50 μM	80.35 ± 1.21**	55.81 ± 2.99***	108.90 ± 0.39*	100.66 ± 5.24	73.22 ± 0.91**
AA 100 μM	60.08 ± 4.04***	76.32 ± 3.80**	99.17 ± 1.07	98.22 ± 1.32	65.50 ± 1.28**
ALA 50 μM	93.14 ± 1.66*	112.27 ± 6.82*	56.41 ± 5.44***	57.14 ± 4.58***	75.00 ± 3.90**
ALA150μM	70.59 ± 1.51**	160.86 ± 7.10***	48.72 ± 6.92***	45.71 ± 7.70***	83.75 ± 6.54**
EPA 50 μM	83.36 ± 6.65**	90.84 ± 6.30*	88.50 ± 0.75*	97.77 ± 2.13	80.41 ± 3.29**
EPA 100 μM	77.83 ± 5.55**	76.98 ± 4.97**	89.54 ± 1.22*	101.33 ± 5.59	81.13 ± 1.00**
DHA 50 μM	88.94 ± 6.91*	80.99 ± 2.88**	81.43 ± 1.09**	67.64 ± 1.95**	69.14 ± 2.85**
DHA100μM	75.88 ± 3.55**	67.00 ± 6.24**	76.84 ± 0.69**	57.71 ± 2.78***	66.69 ± 1.60**