Analysis of aortic atherosclerosis. Representative photographs of pinned-out aortic arch from each group (left, top). Scatter plot of the aortic atherosclerotic lesions on the surface was quantified using an image analysis system (left, bottom). Representative micrographs of the aortic arch from each group (right, top). Serial frozen sections of aortas were stained with hematoxylin-eosin (HE), elastica van Gieson (EVG), or immunohistochemically stained with mAb against macrophages (Mϕ). Intimal microscopic lesions on EVG-stained sections (right, middle) and Mϕ-positive area (right, bottom) were quantified with an image analysis system. The values are expressed as mean ± SEM (n = 8, each group). *P < 0.05, **P < 0.01 vs. vehicle.