Figure 5
Relative expression of c-caspase-3, c-caspase-9, p-p38 MAPK, p-JNK and CD36. (A) Typical western blot bands of c-caspase-3, c-caspase-9, p-p38 MAPK and p-JNK. (B) Relative density of c-caspase-3, c-caspase-9, p-p38 MAPK and p-JNK. (C) Typical western blot bands and relative density of CD36. RAW264.7 macrophages were seeded onto 96-well microtitre plates and supplemented with ox-LDL alone or along with beta2-GPI or reduced beta2-GPI. Total protein was measured with a Micro BCATM Protein Assay Reagent Kit. Aliquots of the total protein were resolved by 4–12% Bis-Tris Gel and blotted onto a nitrocellulose transfer membrane. The membrane was incubated with specific primary antibodies and then with HRP-anti-rabbit IgG. Radiography was performed using a UVP analytical instrument. β-actin was selected as the internal control. Ox-LDL increased the expression of c-caspase-3, c-caspase-9, p-p38 MAPK, p-JNK and CD36 in macrophages. Both beta2-GPI and reduced beta2-GPI inhibited the ox-LDL-induced increase of c-casepase-3, c-casepase-9, p-JNK and CD36, and the latter also inhibited the expression of p-p38 MAPK. Note: n = 3, *: compared with the control group, p < 0.01; ▲: compared with the ox-LDL group, p < 0.05; ▲▲: compared with the ox-LDL group, p < 0.01; #: compared with the beta2-GPI group, p < 0.05.