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Table 2 Weight % of fatty acids in dried blood spots after BHT treatment and storage by test tube or vacuum sealing

From: Butylated hydroxytoluene can protect polyunsaturated fatty acids in dried blood spots from degradation for up to 8 weeks at room temperature

  Week 0* Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8
  Test tube sealed
SFAs 37.2 ± 0.5 37.6 ± 1.1 38.1 ± 1.4 39.1 ± 0.9 36.4 ± 1.2 39.8 ± 5.2 37.3 ± 0.7 36.7 ± 0.2 41.2 ± 2.3
MUFAs 24.6 ± 0.3 25.5 ± 0.5 25.2 ± 0.7 24.1 ± 0.2 25.1 ± 0.8 24.7 ± 2.1 25.6 ± 0.8 25.7 ± 0.3 23.9 ± 1.3
N-6 FAs 29.2 ± 0.2 29.7 ± 0.4 29.0 ± 0.4 28.3 ± 0.7 29.3 ± 1.4 29.3 ± 2.8 30.2 ± 0.6 29.8 ± 0.6 28.2 ± 1.7
N-3 FAs 5.11 ± 0.12 5.15 ± 0.23 5.36 ± 0.33 4.61 ± 0.33 5.04 ± 0.47 4.86 ± 0.43 4.88 ± 0.32 4.92 ± 0.39 4.60 ± 0.39
ARA 6.45 ± 0.04 6.08 ± 0.21 6.12 ± 0.50 6.38 ± 0.46 6.97 ± 0.71 6.87 ± 0.25 6.83 ± 0.54 6.88 ± 0.57 6.51 ± 0.69
EPA 0.86 ± 0.06 0.85 ± 0.05 0.80 ± 0.06 0.86 ± 0.05 0.85 ± 0.11 0.85 ± 0.14 0.86 ± 0.04 0.87 ± 0.06 0.81 ± 0.07
DHA 1.64 ± 0.04 1.48 ± 0.07 1.55 ± 0.18 1.69 ± 0.11 1.93 ± 0.23 1.78 ± 0.05 1.79 ± 0.22 1.80 ± 0.21 1.66 ± 0.18
PUFAs 34.3 ± 0.1 34.8 ± 0.6 34.3 ± 0.7 32.9 ± 1.0 34.4 ± 1.9 34.2 ± 3.2 35.1 ± 0.6 34.7 ± 1.0 32.8 ± 2.0
HUFAs 11.6 ± 0.2 11.1 ± 0.4 11.1 ± 0.9 11.7 ± 0.8 12.9 ± 1.3 12.5 ± 0.5 12.5 ± 1.0 12.6 ± 1.0 11.9 ± 1.2
  Test tube sealed under nitrogen
SFAs 37.2 ± 0.5 37.3 ± 1.4 35.4 ± 0.3 37.7 ± 1.1 36.0 ± 0.2 36.3 ± 1.1 35.4 ± 0.4 37.1 ± 0.8 36.3 ± 1.4
MUFAs 24.6 ± 0.3a 25.3 ± 0.5ab 26.3 ± 0.4bcd 26.0 ± 0.2bcd 26.5 ± 0.2bcd 25.8 ± 1.2abc 26.7 ± 0.2bcd 26.9 ± 0.7cd 27.4 ± 0.5d
N-6 FAs 29.2 ± 0.2ab 29.7 ± 0.6abc 29.9 ± 0.5abc 28.7 ± 0.5a 30.5 ± 0.4abc 30.6 ± 0.7bc 31.1 ± 0.4c 29.7 ± 0.6abc 29.4 ± 1.2abc
N-3 FAs 5.11 ± 0.12ab 5.29 ± 0.04a 5.44 ± 0.11a 4.27 ± 0.09d 4.61 ± 0.14cd 5.10 ± 0.30ab 4.82 ± 0.18bc 4.37 ± 0.12d 4.41 ± 0.18cd
ARA 6.45 ± 0.04abc 6.25 ± 0.08ab 6.13 ± 0.28ab 5.96 ± 0.15a 6.37 ± 0.16ab 7.04 ± 0.49c 6.68 ± 0.19bc 6.07 ± 0.16ab 6.28 ± 0.21ab
EPA 0.86 ± 0.06abc 0.81 ± 0.02abc 0.80 ± 0.06abc 0.76 ± 0.04a 0.84 ± 0.05abc 0.91 ± 0.01c 0.89 ± 0.04bc 0.78 ± 0.03a 0.78 ± 0.05ab
DHA 1.64 ± 0.04ab 1.60 ± 0.04a 1.52 ± 0.13a 1.47 ± 0.05a 1.56 ± 0.04a 1.86 ± 0.22b 1.66 ± 0.08ab 1.46 ± 0.05a 1.51 ± 0.05a
PUFAs 34.3 ± 0.1abc 35.0 ± 0.6abc 35.3 ± 0.6bc 33.0 ± 0.6a 35.1 ± 0.5bc 35.7 ± 0.4bc 35.9 ± 0.6c 34.1 ± 0.7abc 33.8 ± 1.4ab
HUFAs 11.6 ± 0.2ab 11.2 ± 0.1ab 11.2 ± 0.4ab 10.6 ± 0.3a 11.5 ± 0.3ab 12.9 ± 1.0c 12.1 ± 0.4bc 11.0 ± 0.3ab 11.3 ± 0.4ab
Vacuum sealed
SFAs 37.2 ± 0.5 36.5 ± 0.3 37.6 ± 1.1 37.5 ± 1.7 36.7 ± 0.5 35.3 ± 0.6 35.6 ± 0.5 36.7 ± 0.9 36.9 ± 2.2
MUFAs 24.6 ± 0.3a 26.3 ± 0.1b 26.7 ± 0.5b 26.0 ± 0.4ab 26.7 ± 1.0bc 27.8 ± 0.2c 28.1 ± 0.6c 27.8 ± 0.6c 28.0 ± 0.5c
N-6 FAs 29.2 ± 0.2 29.6 ± 0.4 28.6 ± 1.2 28.7 ± 0.6 28.2 ± 1.7 30.0 ± 0.1 29.1 ± 0.7 29.1 ± 0.6 28.5 ± 1.7
N-3 FAs 5.11 ± 0.12a 5.30 ± 0.16a 5.17 ± 0.07a 4.54 ± 0.19b 4.30 ± 0.20b 4.59 ± 0.07b 4.38 ± 0.20b 4.29 ± 0.06b 4.18 ± 0.32b
ARA 6.45 ± 0.04a 6.23 ± 0.16ab 5.49 ± 0.57b 6.15 ± 0.19ab 5.84 ± 0.29ab 6.28 ± 0.07ab 6.04 ± 0.20ab 6.00 ± 0.08ab 5.87 ± 0.40ab
EPA 0.86 ± 0.06a 0.78 ± 0.02ab 0.77 ± 0.05ab 0.80 ± 0.04ab 0.76 ± 0.06ab 0.81 ± 0.02ab 0.76 ± 0.04ab 0.73 ± 0.01b 0.72 ± 0.06b
DHA 1.64 ± 0.04a 1.58 ± 0.05abc 1.36 ± 0.14c 1.60 ± 0.08ab 1.46 ± 0.08abc 1.58 ± 0.05abc 1.51 ± 0.06abc 1.46 ± 0.01abc 1.41 ± 0.10ab
PUFAs 34.3 ± 0.1 34.9 ± 0.5 33.8 ± 1.1 33.3 ± 0.8 32.5 ± 1.9 34.6 ± 0.2 33.5 ± 0.9 33.5 ± 0.6 32.7 ± 2.0
HUFAs 11.6 ± 0.2a 11.3 ± 0.3ab 10.1 ± 1.0b 11.2 ± 0.4ab 10.6 ± 0.6ab 11.5 ± 0.2ab 11.0 ± 0.4ab 10.8 ± 0.2ab 10.6 ± 0.7ab
Nitrogen dried vacuum sealed
SFAs 37.2 ± 0.5 34.8 ± 0.9 35.9 ± 0.1 37.3 ± 1.2 37.6 ± 1.7 35.7 ± 0.5 36.0 ± 1.5 37.9 ± 3.2 35.4 ± 0.7
MUFAs 24.6 ± 0.3a 27.2 ± 0.1bcd 27.0 ± 0.2bc 26.6 ± 0.7b 27.0 ± 0.4bc 28.1 ± 0.3de 27.7 ± 0.5cd 28.2 ± 0.3de 28.7 ± 0.3e
N-6 FAs 29.2 ± 0.2 30.1 ± 0.5 29.4 ± 0.1 28.7 ± 0.9 28.4 ± 0.9 29.7 ± 0.2 29.1 ± 0.8 27.7 ± 2.3 29.5 ± 0.3
N-3 FAs 5.11 ± 0.12a 5.10 ± 0.17a 5.10 ± 0.29a 4.41 ± 0.10b 4.31 ± 0.14b 4.46 ± 0.11ab 4.38 ± 0.21b 4.01 ± 0.50b 4.34 ± 0.13b
ARA 6.45 ± 0.04 6.24 ± 0.22 6.03 ± 0.03 6.02 ± 0.26 5.98 ± 0.19 6.16 ± 0.11 6.06 ± 0.29 5.70 ± 0.54 6.12 ± 0.14
EPA 0.86 ± 0.06a 0.80 ± 0.04ab 0.76 ± 0.02ab 0.77 ± 0.02ab 0.73 ± 0.04b 0.77 ± 0.02ab 0.75 ± 0.04ab 0.68 ± 0.08b 0.75 ± 0.03ab
DHA 1.64 ± 0.04 1.56 ± 0.10 1.49 ± 0.02 1.50 ± 0.09 1.49 ± 0.04 1.52 ± 0.06 1.52 ± 0.07 1.37 ± 0.16 1.47 ± 0.04
PUFAs 34.3 ± 0.1 35.2 ± 0.6 34.5 ± 0.2 33.1 ± 1.0 32.7 ± 1.0 34.2 ± 0.3 33.5 ± 1.0 31.7 ± 2.8 33.8 ± 0.5
HUFAs 11.6 ± 0.2 11.3 ± 0.5 10.9 ± 0.1 11.0 ± 0.4 10.8 ± 0.4 11.2 ± 0.2 11.0 ± 0.5 10.1 ± 1.4 11.1 ± 0.3
  1. Values are means ± SD, n = 3. *Week 0 data baseline is the same analysis for all four storage conditions. SFA, saturated fatty acid; MUFA, monounsaturated fatty acid; FA, fatty acid; n-6, omega-6; n-3, omega-3; ARA, arachidonic acid; EPA, eicosapentaenoic acid; DHA, docosahexaenoic acid; PUFA, polyunsaturated fatty acid; HUFA, highly unsaturated fatty acid. Values with different superscript letters within a row are significantly different by Tukey’s HSD post hoc procedure (P < 0.05) after a significant F-value by one-way ANOVA (P < 0.05).