Figure 2
From: MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRα
MiR-613 directly targets LXRα 3′-UTR. A: A putative binding site of miR-613 in the 3′-UTR of LXRα mRNA and mutated nucleotide residues are shown in red and italics. B: pMIR/LXRαMIRE or pMIR/LXRαMIRE-mut was cotransfected with 80 nM miR-613 mimic or NC into HepG2 cells. C, pMIR-LXRβ was transiently cotransfected with 80 nM indicated RNA oligonucleotides into HepG2 cells. Luciferase assay was conducted after 24 hours. The data was the firefly luciferase activities normalized with the β-galactosidase activities. *, P < 0.05 (n = 3 for each group).