Figure 4
From: MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRα
MiR-613 represses lipogenic genes by mediating LXRα. HepG2 cells were cotransfected with 80 nM miR-613 mimic or NC and LXRα-expressing plasmid pEX-LXRα (without 3′-UTR in LXRα mRNA) for 24 hours. The SREBP-1c, FAS, ChREBP and ACC expression was normalized to β-actin mRNA expression, respectively. The relative level of indicated gene expression determined using the 2-△△CT method. *, P<0.05 (n=3 for each group).