Table 4 Primers used for the Q-PCR assay
From: Gene expression profiling reveals distinct features of various porcine adipose tissues
Gene symbol | Primer sequence (5′ → 3′) | Amplicon length (bp) | GenBank No. |
|---|---|---|---|
ACTB* | F: TCTGGCACCACACCTTCT | 114 | DQ178122 |
R: TGATCTGGGTCATCTTCTCAC | |||
TBP* | F: GATGGACGTTCGGTTTAGG | 124 | DQ178129 |
R: AGCAGCACAGTACGAGCAA | |||
TOP2B* | F: AACTGGATGATGCTAATGATGCT | 137 | AF222921 |
R: TGGAAAAACTCCGTATCTGTCTC | |||
ACADL | F: GTAAGAACAAATGCCAAGA | 103 | NM_213897 |
R: CAGCCACTACAATCACAAC | |||
ADIPOQ | F: GGGTCACTGTCCCTAAC | 209 | NM_214370 |
R: GTCCTGGTACTGGTCGT | |||
ADIPOR1 | F: TGGCTGAAGGACAACGAC | 228 | NM_001007193 |
R: CAAGAAGAACATCCCAAACAC | |||
CAV3 | F: GCATCAGCCATATCTACTCACT | 107 | NM_001037149 |
R: CACTTCTTTCCGCAGCAT | |||
MDH1 | F: TAAGGTTATCGTGGTGGG | 124 | U44846 |
R: TGCTTTAGCTCGGTTGTG | |||
MDH2 | F: CGAGGTGGTCAAGGCTAAG | 172 | M16427 |
R: CAATGGCGTGGAGAAATAC | |||
ME 1 | F: GTTGCCCTTGGTGTTGT | 212 | X93016 |
R: GGATAAATGGTGGCTGTC | |||
Insig1 | F: TCAACCACGCCAGTGCTAA | 251 | AY336601.2 |
R: CACGCCTCCCGAGAAGAAA | |||
SCAP | F: CGCCTGAAACAGAAATCG | 213 | XM_001924223 |
R: TCTCCTGAGCCTCCAACA |