Phenotypic analysis of T-helper cells in mesenteric lymph nodes at 4 weeks after AT. Ex-vivo T cell stimulation, fixation and staining were performed then analyzed by flow-cytometry. Representative flow-cytometry dot plot pictures and gating strategy to quantify% of Th1, Th17 and regulatory T cells (A). Note the clear differences in each subset frequency between a healthy mice and colitic mice irrespectively of the diet used.% of Th1, Th17 cells and regulatory T cells are shown in colitic mice (tRag2 and tRag2 + FO) (B). Black bars are median values and each dot represents the value obtained with an individual mouse.