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Table 1 The oligo sequences of four FASN specific RNAi plasmids and the negative control RNAi plasmid

From: Loss of fatty acid synthase inhibits the “HER2-PI3K/Akt axis” activity and malignant phenotype of Caco-2 cells

Plasmid

 

Oligo sequence (5′-3′)

MR-FASN-1

Forward

TGCTGAACTCCTGCAAGTTCTCCGACGTTTTGGCCACTGACTGACGTCGGAGATTGCAGGAGTT

 

Reverse

CCTGAACTCCTGCAATCTCCGACGTCAGTCAGTGGCCAAAACGTCGGAGAACTTGCAGGAGTTC

MR-FASN-2

Forward

TGCTGAGAAGAGGCTCTCCCACGCGTGTTTTGGCCACTGACTGACACGCGTGGGAGCCTCTTCT

 

Reverse

CCTGAGAAGAGGCTCCCACGCGTGTCAGTCAGTGGCCAAAACACGCGTGGGAGAGCCTCTTCTC

MR-FASN-3

Forward

TGCTGATACCTAGCAGGCTGTCCTGGGTTTTGGCCACTGACTGACCCAGGACACTGCTAGGTAT

 

Reverse

CCTGATACCTAGCAGTGTCCTGGGTCAGTCAGTGGCCAAAACCCAGGACAGCCTGCTAGGTATC

MR-FASN-4

Forward

TGCTGTAGCCAAGCACCTCACGCTGGGTTTTGGCCACTGACTGACCCAGCGTGGTGCTTGGCTA

 

Reverse

CCTGTAGCCAAGCACCACGCTGGGTCAGTCAGTGGCCAAAACCCAGCGTGAGGTGCTTGGCTAC

MR-Neg

Forward

tgctgAAATGTACTGCGCGTGGAGACGTTTTGGCCACTGACTGACGTCTCCACGCAGTACATTT

 

Reverse

cctgAAATGTACTGCGTGGAGACGTCAGTCAGTGGCCAAAACGTCTCCACGCGCAGTACATTTc

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Lipids in Health and Disease

ISSN: 1476-511X