In vivo and in vitro Cu2+-catalyzed oxidation of LDL and its sd-LDL and lb-LDL subfractions from plasma of hyperlipidemic rats after 30 days of ME and VO treatment. Here, expression of the conjugated diene (CD) values is as nmole malondialdehyde (MDA) equivalents/mg protein. The basal CD values show the status of oxidized LDL, and its subfractions sd-LDL and lb-LDL in vivo. After 2 h of incubation for lipoproteins, LDL, lb-LDL, and 30 min of incubation for sd-LDL with CuSO4, their maximal CD formation values were determined. The interval between the intercept of the tangent of the slope of the curve with the time in min is known as lag time. Each value represents mean ± SD from pooled plasma samples in each group. The values of HLP-C control groups were statistically significant from NLP-C groups (p < 0.05), the values of treated groups, HLP-ME and HLP-VO were significantly different from HLP-C groups (p < 0.05), and the values between the treated groups were also significant (p < 0.05).