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Figure 5 | Lipids in Health and Disease

Figure 5

From: Autophagy involved in lipopolysaccharide-induced foam cell formation is mediated by adipose differentiation-related protein

Figure 5

ADRP overexpression increases the LPS-induced autophagic activity, while ADRP knockdown downregulates the autophagic activity in THP-1 macrophages. (A) ADRP overexpression by adenoviral gene transfer augmented LPS-induced LC3 protein levels as determined by western blotting and the percentage of cells with autophagosomes were calculated. (B) Specific knockdown of ADRP with ADRP siRNA prior to LPS stimulation of THP-1 cells inhibited the LC3-II/I ratio as determined by western blotting and the percentage of cells with autophagosomes was calculated. Results were presented as mean ± SEM of 3 independent experiments, each in triplicate. *significant difference from LPS treatment P < 0.05; **, P < 0.01.

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Lipids in Health and Disease

ISSN: 1476-511X