Figure 2

Protein and mRNA Expression profiles of components of the HSR in non-diabetic obese subjects. (A) Representative slides of HSPs and chaperone expression levels using IHC and the subcutaneous adipose tissue from lean and obese participants (n = 10 for each group). Aperio software was used to quantify positive staining from lean and obese participants as detailed in Material and Methods. (B) Representative Western blots of lean and obese subjects showing the expression pattern of various HSPs and chaperones. Total proteins were extracted from PBMCs of lean and obese participants (n = 9 for each group) and detected using the indicated antibodies. The blots shown are representative of 3 experiments with consistent results. Densitometric quantification of the Western blots data. The bands of interest were quantified as described in materials and methods and the relative intensities were determined after correction with Actin. (C) Quantitative analysis of HSPs mRNA in lean and obese subjects using PBMCs (n = 9 for each group). Total mRNA was isolated and subjected to analysis using qRT-PCR. The quantified data are presented as fold changes in obese compared to lean subjects. Non-parametric Mann–Whitney test was used for statistical analysis.