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Table 1 RT 2 profiler PCR array, lipoprotein signaling and cholesterol metabolism

From: ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?

Gene Description PXE/control siABCC6/siNK
Fold changea p-value ( t-test)b Commentsc Fold changea p-value ( t-test)b Commentsc
ABCG1 ATP-binding cassette, sub-family G member 1 1.89 0.334 B 0.34 0.421 B
ANGPTL3 Angiopoietin-like 3 3.09 0.249 A 1.24 0.294 A
APOD Apolipoprotein D 2.29 0.328 OKAY 0.92 0.796 OKAY
APOE Apolipoprotein E 0.07 0.313 A 0.51 0.847 A
APOF Apolipoprotein F 0.47 0.353 B 1.90 0.343 B
APOL1 Apolipoprotein L, 1 124.57 0.095 A 1.03 0.919 B
APOL5 Apolipoprotein L, 5 0.23 0.123 B 1.05 0.660 C
CEL Carboxyl ester lipase (bile salt-stimulated lipase) 2.68 0.341 A 2.84 0.331 A
CXCL16 Chemokine (C-X-C motif) ligand 16 0.49 0.192 B 1.09 0.869 OKAY
CYP39A1 Cytochrome P450, family 39, subfamily A, polypeptide 1 3.64 0.068 A 1.05 0.809 B
CYP46A1 Cytochrome P450, family 46, subfamily A, polypeptide 1 0.57 0.250 B 0.47 0.192 B
HDLBP High density lipoprotein binding protein 6.99 0.751 OKAY 0.99 0.989 OKAY
LRP1B Low density lipoprotein receptor-related protein 1B 5.18 0.092 B 0.43 0.265 B
NPC1L1 NPC1 (Niemann-Pick disease, type C1, gene) like 1 0.36 0.161 B 0.89 0.562 B
OLR1 Oxidized low density lipoprotein (lectin-like) receptor 1 0.29 0.120 B 2.34 0.581 B
PCSK9 Proprotein convertase subtilisin/kexin type 9 46.23 0.033 A 1.18 0.820 B
SORL1 Sortilin-related receptor, L(DLR class) A repeats containing 1.77 0.112 B 2.18 0.390 B
TM7SF2 Transmembrane 7 superfamily member 2 2.40 0.034 OKAY 2.05 0.079 OKAY
TRERF1 Transcriptional regulating factor 1 4.93 0.222 OKAY 3.98 0.209 OKAY
  1. Regulated gene expression of human dermal fibroblasts of PXE patients and healthy controls (PXE/controls) and siRNA transfected cells (siABCC6/siNK) cultivated without FCS for 24 h.
  2. afold-Change (2^(−ΔΔCt)) is the normalized gene expression (2^(−ΔCt)) in the test sample divided the normalized gene expression (2^(−ΔCt)) in the control sample.
  3. bp-values are calculated based on a Student’s t-test of the replicate 2^(−ΔCt) values for each gene in the control group and treatment groups, and p values less than 0.05 are printed in bold.
  4. ccomments
  5. OKAY: This gene's average threshold cycle is below 30 in the control and test samples.
  6. A: This gene’s average threshold cycle is relatively high (>30) in either the control or the test sample, and is reasonably low in the other sample (<30).
  7. B: This gene’s average threshold cycle is relatively high (>30), meaning that its relative expression level is low, in both control and test samples, and the p-value for the fold-change is either unavailable or relatively high (p > 0.05).
  8. C: This gene’s average threshold cycle is either not determined or greater than the defined cut-off value (default 35), in both samples meaning that its expression was undetected, making this fold-change result erroneous and un-interpretable.