ABCC6- a new player in cellular cholesterol and lipoprotein metabolism?

Table 1 RT ² profiler PCR array, lipoprotein signaling and cholesterol metabolism

Gene	Description	PXE/control			siABCC6/siNK
Gene	Description	Fold change^a	p-value ( t-test)^b	Comments^c	Fold change^a	p-value ( t-test)^b	Comments^c
ABCG1	ATP-binding cassette, sub-family G member 1	1.89	0.334	B	0.34	0.421	B
ANGPTL3	Angiopoietin-like 3	3.09	0.249	A	1.24	0.294	A
APOD	Apolipoprotein D	2.29	0.328	OKAY	0.92	0.796	OKAY
APOE	Apolipoprotein E	0.07	0.313	A	0.51	0.847	A
APOF	Apolipoprotein F	0.47	0.353	B	1.90	0.343	B
APOL1	Apolipoprotein L, 1	124.57	0.095	A	1.03	0.919	B
APOL5	Apolipoprotein L, 5	0.23	0.123	B	1.05	0.660	C
CEL	Carboxyl ester lipase (bile salt-stimulated lipase)	2.68	0.341	A	2.84	0.331	A
CXCL16	Chemokine (C-X-C motif) ligand 16	0.49	0.192	B	1.09	0.869	OKAY
CYP39A1	Cytochrome P450, family 39, subfamily A, polypeptide 1	3.64	0.068	A	1.05	0.809	B
CYP46A1	Cytochrome P450, family 46, subfamily A, polypeptide 1	0.57	0.250	B	0.47	0.192	B
HDLBP	High density lipoprotein binding protein	6.99	0.751	OKAY	0.99	0.989	OKAY
LRP1B	Low density lipoprotein receptor-related protein 1B	5.18	0.092	B	0.43	0.265	B
NPC1L1	NPC1 (Niemann-Pick disease, type C1, gene) like 1	0.36	0.161	B	0.89	0.562	B
OLR1	Oxidized low density lipoprotein (lectin-like) receptor 1	0.29	0.120	B	2.34	0.581	B
PCSK9	Proprotein convertase subtilisin/kexin type 9	46.23	0.033	A	1.18	0.820	B
SORL1	Sortilin-related receptor, L(DLR class) A repeats containing	1.77	0.112	B	2.18	0.390	B
TM7SF2	Transmembrane 7 superfamily member 2	2.40	0.034	OKAY	2.05	0.079	OKAY
TRERF1	Transcriptional regulating factor 1	4.93	0.222	OKAY	3.98	0.209	OKAY

Regulated gene expression of human dermal fibroblasts of PXE patients and healthy controls (PXE/controls) and siRNA transfected cells (siABCC6/siNK) cultivated without FCS for 24 h.
^afold-Change (2^(−ΔΔC_t)) is the normalized gene expression (2^(−ΔC_t)) in the test sample divided the normalized gene expression (2^(−ΔC_t)) in the control sample.
^bp-values are calculated based on a Student’s t-test of the replicate 2^(−ΔC_t) values for each gene in the control group and treatment groups, and p values less than 0.05 are printed in bold.
^ccomments
OKAY: This gene's average threshold cycle is below 30 in the control and test samples.
A: This gene’s average threshold cycle is relatively high (>30) in either the control or the test sample, and is reasonably low in the other sample (<30).
B: This gene’s average threshold cycle is relatively high (>30), meaning that its relative expression level is low, in both control and test samples, and the p-value for the fold-change is either unavailable or relatively high (p > 0.05).
C: This gene’s average threshold cycle is either not determined or greater than the defined cut-off value (default 35), in both samples meaning that its expression was undetected, making this fold-change result erroneous and un-interpretable.

ISSN: 1476-511X