Table 1 List of RT-PCR primers
From: The involvement of high mobility group 1 cytokine and phospholipases A2 in diabetic retinopathy
Gene | Forward | Reverse |
|---|---|---|
β-Actin | 5′-CGT AAA GAC CTC TAT GCC AA-3′ | 5′-AGC CAT GCC AAA TGT GTC AT-3′ |
TNF-α | 5′-CAA AAT TCG AGT GAC AAG CCT G-3′ | 5′-GAG ATC CAT GCC GTT GGC-3′ |
VEGF | 5′-AAC CAT GAA CTT TCT GCT CTC TTG-3′ | 5′-GCC TGG CTC ACC GCCTTG GCT TGT C-3′ |
ICAM-1 | 5′-CCC TGT CAG TCC GGA AAT AA-3′ | 5′-GAT GAC TTT TGA GGG GGA CA-3′ |
PLA2 | 5′-CAG CAA GGA UCC UCG CUA U-3′ | 5′-CAG AAU GCU UCC AAU CGU A-3′ |