Table 3 Primers used in real-time PCR analysis
Gene | Primer sequence | Size (bp) | Tm (°C) |
|---|---|---|---|
VLDL receptor | (F)ACCTCTGGCCAAATATGCAC | 304 | 58.9 |
(R)CACTCAGTCTTTGCAAACCTCC | |||
LDL receptor | (F)GCGTCCCTGTACAGATAGTGG | 284 | 63.3 |
(R)GCCACTCATACATACAACGG | |||
HMG-CoA reductase | (F)CCACGAACGCTCTTAGCTTTC | 215 | 57.1 |
(R)CTAAGAGGCTCTCCATGCTGC | |||
β-actin | (F)GGATGCAGAAGGAGATCACTG | 90 | |
(R)CGATCCACACGGAGTACTTG |