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Table 1 Composition of the control diet, control diet supplemented with oligofructose, diet enriched with trans fatty acids and diet enriched with trans fatty acids supplemented with oligofructose according to AIN-93

From: Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups

    Diet (g/100 g)  
Ingredient C CF T TF
Casein* 20.0 20.0 20.0 20.0
L-cystine† 0.3 0.3 0.3 0.3
Cornstarch† 62.0 52.0 62.0 52.0
Soybean oil‡ 8.0 8.0 1.0 1.0
Hydrogenated vegetablefat$ - - 7.0 7.0
Butylhydroquinone† 0.0014 0.0014 0.0014 0.0014
Mineral mixture§ 3.5 3.5 3.5 3.5
Vitamin mixture# 1.0 1.0 1.0 1.0
Cellulose† 5.0 5.0 5.0 5.0
Choline bitartrate† 0.25 0.25 0.25 0.25
Oligofructose£ - 10.0 - 10.0
Energy (kcal/g) 4.0 4.0 4.0 4.0
  1. *Casein was obtained from Labsynth, São Paulo, Brazil.
  2. †L-cystine, cornstarch, butylhydroquinone, cellulose and choline bitartrate were obtained from Viafarma, São Paulo, Brazil.
  3. ‡Oil was supplied from soybean (Lisa/Ind. Brazil).
  4. $Hydrogenated vegetable fat was supplied from Unilever, São Paulo, Brazil.
  5. §Mineral mix 9 mg/kg diet): calcium, 5000; phosphorus, 1561; potassium, 3600;sodium, 1019; chloride, 1571; sulfur, 300; magnesium, 507; iron, 35; copper, 6.0; manganese, 10.0; zinc, 30.0; chromium, 1.0; iodine 0.2; selenium, 0.15; fluoride, 1.00; boron, 0.50; molybdenum, 0.15; silicon, 5.0; nickel, 0.5; lithium, 0.1; vanadium, 0.1 (AIN-93G, mineral mix, Rhoster, Brazil).
  6. #Vitamin mix (mg/kg diet): thiamin HCL, 6.0, riboflavin, 6.0; pyridoxine HCL 7.0;niacin, 30.0; calcium pantothenate, 16.0; folic acid, 2.0; biotin, 0.2; vitamin B12,25.0; vitamin A palmitate 4000 IU; vitamin E acetate, 75; vitamin D3, 1000 IU; vitamin KI, 0.75. (AIN-93G, vitamin mix, Rhoster, Brazil).
  7. £Oligofructose (P95) was manufactured by Orafti (Pemuco, Chile) and was obtained by Viafarma, São Paulo, Brazil.