Aortic wall thickness and wrinkling of elastic layers. Aortic wall thickness and wrinkling of elastic layers. Venhoeff & Van Gienson stain. (400x). Images on the left: Wistar rats. Images on the right: SHROB rats. (A and B) EPA:DHA 1:1 treatment. (C and D) EPA:DHA 2:1 treatment. (E and F) EPA:DHA 1:2 treatment. As we can see, images on the right, are thicker and less dense, and have less wrinkling, therefore are less functional.