Figure 1

Effect of DHC on apoM expression in HepG2 cells. (A and B) HepG2 cells were divided into four groups and cultured in medium at 37°C containing 0 μM, 25 μM, 50 μM and 100 μM DHC for 24 h, respectively. ApoM transcript levels and protein levels were measured by real-time quantitative PCR and western blot analysis, respectively. (C and D) HepG2 cells were treated with 100 μM DHC for 0, 6, 12, and24 h, respectively. ApoM transcript levels and protein levels were measured by real-time quantitative PCR and western blot analysis, respectively. All results are presented as mean ± SD of three independent experiments, each performed in triplicate. *P < 0.05 vs. the control group.