Table 1 Differentially expressed proteins and mRNA abundances of genes in the small intestinal mucosa of VDR-KO relative to WT mice
UniProtAcc. | Protein | Spot no. | Fold changea | No. of peptides | Coverage (%) | pI (theor.) | Mascot score | mRNA fold changea | Function |
|---|---|---|---|---|---|---|---|---|---|
P14206 | 37/67 kDa laminin receptor (37/67LR) | 16 | +10.8 | 9 | 39 | 4.80 | 422 | n.s. | Membrane receptor for laminin |
P08113 | 94 kDa glucose-regulated protein (GRP94) | 18, 19 | +5.0, +5.5 | 22 | 28 | 4.74 | 307 | -1.4* | Chaperone, stress response |
P21107 | Tropomyosin 3 (Tpm3) | 13 | +4.3 | 11 | 28 | 4.68 | 225 | n.d. | Cytoskeleton |
Q04857 | Collagen type VI (alpha 1 chain) (Col6a1) | 1 | +4.2 | 9 | 10 | 5.20 | 88 | +1.8* | Extracellular matrix protein |
Q01853 | Valosin-containing protein(VCP/p97) | 17 | +3.8 | 18 | 28 | 5.14 | 204 | n.s. | Chaperon, stress response, protein degradation |
P97372 | Proteasome activator complex subunit 2 (PA28β) | 8 | +3.1 | 8 | 40 | 5.54 | 35c | -1.6* | Proteasome activation |
P19001 | Keratin, type I cytoskeletal 19 (K19) | 14, 15 | +2.1, +2.9 | 14 | 39 | 5.28 | 127 | n.s. | Cytoskeleton |
Q60604 | Adseverin | 2 | +2.8 | 18 | 30 | 5.46 | 88 | n.s. | Cytoskeleton |
P60710 | β-actin | 5, 6, 7 | -6.5, -4.2-4.1 | 5 | 15-21 | 5.29b | 54-70 | -2.0* | Cytoskeleton |
Q9CQ60 | 6-phosphogluconolactonase (6PGL) | 9 | -2.7 | 3 | 15 | 5.55 | 39c | n.s. | Carbohydrate metabolism |
P63017 | Heat shock cognate 71 kDa protein (Hsc70) | 3, 4 | -2.5, -2.6 | 31 | 52 | 5.37 | 586 | n.s. | Chaperone, stress response |
P51162 | Ileal lipid-binding protein (ILBP) | 10 | -2.5 | 8 | 68 | 5.91 | 210 | n.d. | Lipid metabolism |
P55050 | Fatty acid-binding protein 2 (I-FABP) | 11 | -2.4 | 4 | 31 | 6.62 | 102 | n.s. | Lipid metabolism |
P63101 | 14-3-3 protein ζ/δ | 12 | -2.3 | 18 | 63 | 4.73 | 353 | -1.6* | Signal transduction |