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Table 1 Weight gain, food intake, and brain weight of male control rats and ID + n-3 FAD rats repleted with an Fe + ALA, ID + ALA, Fe + n-3 FAD, or ID + n-3 FAD diet for 5 wk

From: Providing male rats deficient in iron and n-3 fatty acids with iron and alpha-linolenic acid alone affects brain serotonin and cognition differently from combined provision

  p Value
  Control Fe + ALA ID + ALA Fe + n-3 FAD ID + n-3 FAD Fe ALA Fe × ALA
Body weight, g 409 ± 8.0 338 ± 12.5*,Δ,¥ 302 ± 7.4*,# 320 ± 7.8* 290 ± 7.1*,#,‡ 0.001 0.29 0.83
Total body weight gain, g/35d 124 ± 4.4 167 ± 9.4*,Δ,¥ 136 ± 3.8#,‡ 154 ± 4.0*,Δ,¥ 138 ± 4.3#,‡ 0.001 0.43 0.30
Total food intake, g/35d 711 ± 14.3 701 ± 26.1Δ,¥ 624 ± 12.1*,#,‡ 666 ± 12.2Δ,¥ 602 ± 9.0*,#,‡ <0.001 0.14 0.78
Relative weight gain, g/g food intake 0.17 ± 0.01 0.24 ± 0.01* 0.22 ± 0.01*,# 0.23 ± 0.00* 0.23 ± 0.00* 0.20 0.98 0.14
  1. Values are means ± SEM, n = 8–10 per group. Abbreviations: ALA, alpha-linolenic acid; n-3 FAD, n-3 fatty acid deficient; Fe, iron; ID, iron deficient.
  2. p Values correspond to two-way ANOVA to test effects of dietary Fe (deficient vs. sufficient) and dietary ALA (deficient vs. sufficient), and Fe × ALA interactions.
  3. *P <0.05 versus control group, analyzed using 1-way ANOVA with diet as between-subject variable followed by post hoc Dunnett’s test to determine whether experimental (repletion) groups differed from age-matched positive-control rats after repletion with Fe and/or ALA.
  4. #P <0.05 versus Fe + ALA group; ΔP <0.05 versus ID + ALA group; P <0.05 versus Fe + n-3 FAD group; ¥P <0.05 versus ID + n-3 FAD group; Analyzed using 1-way ANOVA with diet (excluding control group) as between-subject variable followed by post hoc Tukey’s test to determine differences between experimental (repletion) groups.