Providing male rats deficient in iron and n-3 fatty acids with iron and alpha-linolenic acid alone affects brain serotonin and cognition differently from combined provision

Baumgartner, Jeannine; Smuts, Cornelius M; Zimmermann, Michael B

doi:10.1186/1476-511X-13-97

Lipids in Health and Disease

Table 2 Iron concentration and major phospholipid fatty acid composition in different brain regions of male control rats and ID + n-3 FAD rats repleted with an Fe + ALA, ID + ALA, Fe + n-3 FAD, or an ID + n-3 FAD diet for 5 weeks

From: Providing male rats deficient in iron and n-3 fatty acids with iron and alpha-linolenic acid alone affects brain serotonin and cognition differently from combined provision

						p Value
	Control	Fe + ALA	ID + ALA	Fe + n-3 FAD	ID + n-3 FAD	Fe	ALA	Fe × ALA
Brain Fe, nmol/g tissue
FC	235 ± 11.2	210 ± 8.8	186 ± 10.5*	208 ± 10.5	190 ± 14.7*	0.064	0.97	0.80
OB	300 ± 21.5	303 ± 23.0^‡,¥,Δ	235 ± 9.3^#	234 ± 12.1*^,#	222 ± 18.7*^,#	0.033	0.009	0.13
Str	263 ± 13.6	239 ± 14.4	223 ± 8.0	237 ± 14.2	204 ± 17.3*	0.12	0.26	0.62
Hip	213 ± 6.7	192 ± 7.0	186 ± 19.8	198 ± 19.4	161 ± 11.9*	0.13	0.97	0.29
Tissue fatty acids, % of total FA
20:5 n-3 (EPA)
FC	0.05 ± 0.01	0.07 ± 0.01	0.08 ± 0.02	0.07 ± 0.01	0.05 ± 0.01	0.60	0.43	0.23
OB	0.08 ± 0.01	0.10 ± 0.02	0.07 ± 0.01	0.08 ± 0.01	0.07 ± 0.01	0.10	0.61	0.32
Str	0.07 ± 0.01	0.04 ± 0.01	0.07 ± 0.01	0.06 ± 0.01	0.06 ± 0.01	0.27	0.59	0.32
Hip	0.20 ± 0.10	0.11 ± 0.02	0.10 ± 0.02	0.14 ± 0.03	0.09 ± 0.03	0.23	0.72	0.40
22:6 n-3 (DHA)
FC	13.5 ± 0.2	7.7 ± 0.2*^,‡,¥	7.7 ± 0.3*^,‡,¥	3.7 ± 0.1*^,#,Δ	4.0 ± 0.2*^,#,Δ	0.51	<0.001	0.53
OB	16.0 ± 0.3	10.1 ± 0.6*^,‡,¥	9.6 ± 0.4*^,‡,¥	4.5 ± 0.3*^,#,Δ	5.2 ± 0.3*^,#,Δ	0.75	<0.001	0.16
Str	14.1 ± 0.3	7.9 ± 0.3*^,‡,¥	7.6 ± 0.2*^,‡,¥	4.5 ± 0.3*^,#,Δ	4.3 ± 0.3*^,#,Δ	0.34	<0.001	0.71
Hip	14.5 ± 0.6	9.7 ± 0.6*^,Δ,‡,¥	7.7 ± 0.2*^,#,‡,¥	4.2 ± 0.3*^,#,Δ	4.7 ± 0.5*^,#,Δ	0.10	<0.001	0.007
Total n-3 FA
FC	14.0 ± 0.2	8.4 ± 0.3*^,‡,¥	8.6 ± 0.3*^,‡,¥	4.0 ± 0.2*^,#,Δ	4.2 ± 0.2*^,#,Δ	0.47	<0.001	0.92
OB	16.5 ± 0.3	10.8 ± 0.6*^,‡,¥	10.2 ± 0.4*^,‡,¥	4.9 ± 0.3*^,#,Δ	5.6 ± 0.3*^,#,Δ	0.89	<0.001	0.13
Str	14.6 ± 0.3	8.5 ± 0.3*^,‡,¥	8.3 ± 0.2*^,‡,¥	4.8 ± 0.3*^,#,Δ	4.7 ± 0.3*^,#,Δ	0.46	<0.001	0.88
Hip	15.5 ± 1.0	10.6 ± 0.5*^,Δ,‡,¥	8.7 ± 0.3*^,#,‡,¥	4.7 ± 0.2*^,#,Δ	5.2 ± 0.5*^,#,Δ	0.10	<0.001	0.007

Values are means ± SEM, n = 8–10 per group. Abbreviations: ALA, alpha-linolenic acid; n-3 FAD, n-3 fatty acid deficient; FC, frontal cortex; Fe, iron; Hip, hippocampus; ID, iron deficient; OB, olfactory bulb; Str, striatum.
p Values correspond to two-way ANOVA to test effects of dietary Fe (deficient vs. sufficient) and dietary ALA (deficient vs. sufficient), and Fe × ALA interactions.
*P <0.05 versus control group, analyzed using 1-way ANOVA with diet as between-subject variable followed by post hoc Dunnett’s test to determine whether experimental (repletion) groups differed from age-matched positive-control rats after repletion with Fe and/or ALA.
^#P <0.05 versus Fe + ALA group; ^ΔP <0.05 versus ID + ALA group; ^‡P <0.05 versus Fe + n-3 FAD group; ^¥P <0.05 versus ID + n-3 FAD group; Analyzed using 1-way ANOVA with diet (excluding control group) as between-subject variable followed by post hoc Tukey’s test to determine differences between experimental (repletion) groups.

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ISSN: 1476-511X

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