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Table 2 Iron concentration and major phospholipid fatty acid composition in different brain regions of male control rats and ID + n-3 FAD rats repleted with an Fe + ALA, ID + ALA, Fe + n-3 FAD, or an ID + n-3 FAD diet for 5 weeks

From: Providing male rats deficient in iron and n-3 fatty acids with iron and alpha-linolenic acid alone affects brain serotonin and cognition differently from combined provision

  p Value
  Control Fe + ALA ID + ALA Fe + n-3 FAD ID + n-3 FAD Fe ALA Fe × ALA
Brain Fe, nmol/g tissue
FC 235 ± 11.2 210 ± 8.8 186 ± 10.5* 208 ± 10.5 190 ± 14.7* 0.064 0.97 0.80
OB 300 ± 21.5 303 ± 23.0‡,¥,Δ 235 ± 9.3# 234 ± 12.1*,# 222 ± 18.7*,# 0.033 0.009 0.13
Str 263 ± 13.6 239 ± 14.4 223 ± 8.0 237 ± 14.2 204 ± 17.3* 0.12 0.26 0.62
Hip 213 ± 6.7 192 ± 7.0 186 ± 19.8 198 ± 19.4 161 ± 11.9* 0.13 0.97 0.29
Tissue fatty acids, % of total FA
20:5 n-3 (EPA)         
FC 0.05 ± 0.01 0.07 ± 0.01 0.08 ± 0.02 0.07 ± 0.01 0.05 ± 0.01 0.60 0.43 0.23
OB 0.08 ± 0.01 0.10 ± 0.02 0.07 ± 0.01 0.08 ± 0.01 0.07 ± 0.01 0.10 0.61 0.32
Str 0.07 ± 0.01 0.04 ± 0.01 0.07 ± 0.01 0.06 ± 0.01 0.06 ± 0.01 0.27 0.59 0.32
Hip 0.20 ± 0.10 0.11 ± 0.02 0.10 ± 0.02 0.14 ± 0.03 0.09 ± 0.03 0.23 0.72 0.40
22:6 n-3 (DHA)
FC 13.5 ± 0.2 7.7 ± 0.2*,‡,¥ 7.7 ± 0.3*,‡,¥ 3.7 ± 0.1*,#,Δ 4.0 ± 0.2*,#,Δ 0.51 <0.001 0.53
OB 16.0 ± 0.3 10.1 ± 0.6*,‡,¥ 9.6 ± 0.4*,‡,¥ 4.5 ± 0.3*,#,Δ 5.2 ± 0.3*,#,Δ 0.75 <0.001 0.16
Str 14.1 ± 0.3 7.9 ± 0.3*,‡,¥ 7.6 ± 0.2*,‡,¥ 4.5 ± 0.3*,#,Δ 4.3 ± 0.3*,#,Δ 0.34 <0.001 0.71
Hip 14.5 ± 0.6 9.7 ± 0.6*,Δ,‡,¥ 7.7 ± 0.2*,#,‡,¥ 4.2 ± 0.3*,#,Δ 4.7 ± 0.5*,#,Δ 0.10 <0.001 0.007
Total n-3 FA
FC 14.0 ± 0.2 8.4 ± 0.3*,‡,¥ 8.6 ± 0.3*,‡,¥ 4.0 ± 0.2*,#,Δ 4.2 ± 0.2*,#,Δ 0.47 <0.001 0.92
OB 16.5 ± 0.3 10.8 ± 0.6*,‡,¥ 10.2 ± 0.4*,‡,¥ 4.9 ± 0.3*,#,Δ 5.6 ± 0.3*,#,Δ 0.89 <0.001 0.13
Str 14.6 ± 0.3 8.5 ± 0.3*,‡,¥ 8.3 ± 0.2*,‡,¥ 4.8 ± 0.3*,#,Δ 4.7 ± 0.3*,#,Δ 0.46 <0.001 0.88
Hip 15.5 ± 1.0 10.6 ± 0.5*,Δ,‡,¥ 8.7 ± 0.3*,#,‡,¥ 4.7 ± 0.2*,#,Δ 5.2 ± 0.5*,#,Δ 0.10 <0.001 0.007
  1. Values are means ± SEM, n = 8–10 per group. Abbreviations: ALA, alpha-linolenic acid; n-3 FAD, n-3 fatty acid deficient; FC, frontal cortex; Fe, iron; Hip, hippocampus; ID, iron deficient; OB, olfactory bulb; Str, striatum.
  2. p Values correspond to two-way ANOVA to test effects of dietary Fe (deficient vs. sufficient) and dietary ALA (deficient vs. sufficient), and Fe × ALA interactions.
  3. *P <0.05 versus control group, analyzed using 1-way ANOVA with diet as between-subject variable followed by post hoc Dunnett’s test to determine whether experimental (repletion) groups differed from age-matched positive-control rats after repletion with Fe and/or ALA.
  4. #P <0.05 versus Fe + ALA group; ΔP <0.05 versus ID + ALA group; P <0.05 versus Fe + n-3 FAD group; ¥P <0.05 versus ID + n-3 FAD group; Analyzed using 1-way ANOVA with diet (excluding control group) as between-subject variable followed by post hoc Tukey’s test to determine differences between experimental (repletion) groups.