Expression of human lipoprotein lipase (hLPL) mRNA in transgenic rabbits. The cDNA obtained by reverse transcription (RT) of total RNA extracted from skeletal muscles or perirenal fat and primed by random primers followed by 35 cycles of PCR with hLPL-specific primers, was loaded on 2% agarose gel. Reaction was performed in parallel in the absence of reverse transcriptase (RT-), to ensure for lack of genomic DNA contamination. Typical RT-PCR results are shown for semimembranosus proprius muscle. Lanes 1–4: RT-PCR product in hLPL rabbit; Lane 5: RT- in hLPL rabbit; Lane 6: 100 bp DNA ladder; Lane 7–10: RT-PCR product in wild-type rabbit; Lane 11: RT- in wild-type rabbit. A band at the expected size of 137 bp was detected in hLPL rabbits only.