Figure 1
Real-time RT-PCR for iNOS mRNA expression in 3T3-Swiss fibroblasts n = 3, ± SD). The expression of iNOS mRNA can be altered by stimulation of the NF-κB pathway or inhibition of nitric oxide synthase in 3T3-Swiss fibroblasts. EPA treated fibroblasts were more sensitive to the changes of NF-κB pathway than AA treated cells. Cells were incubated for 48 hr with bovine serum albumin alone as control or bovine serum albumin-soap loaded fatty acids (25 μM). Medium was then replaced with fresh fatty acid enriched medium containing lipopolysaccharide (LPS, 10 μg/ml) with or without NG-nitro-L-arginine methyl ester (L-NAME, 10-7 M) for another 24 hr. The wound was created after initial 48 hr treatments in duplicate set of plate with or without LPS. Cells were harvested at 24 hr post wounding and real-time RT-PCR was performed. The results were presented as the percentage of iNOS to β-actin mRNA expression. Bars with different letters are significantly different (P < 0.05). EPA, eicosapentaenoic acid; AA, arachidonic acid; LPS, lipopolysaccharide; L-NAME, NG-nitro-L-arginine methyl ester.