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Figure 2 | Lipids in Health and Disease

Figure 2

From: Cell surface heparan sulfate proteoglycans contribute to intracellular lipid accumulation in adipocytes

Figure 2

Differentiated adipocytes bind and internalize more RAP than pre-adipocytes. Pre-adipocyte 3T3-L1 cells and differentiated adipocytes were incubated with 125I-RAP (500 ng/ml) in the presence (hatched bars) or absence (solid bars) of unlabeled RAP (20 μg/ml) at 4°C for 3 h (A) or 37°C for 3 h (B). For 4°C incubations, bound ligand was solubilized and directly quantitated by scintillation counting. For 37°C incubations, culture media was processed for trichloroacetic acid (TCA) precipitation and soluble radioactivity, representing degraded ligand, was quantitated by scintillation counting.

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