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Figure 7 | Lipids in Health and Disease

Figure 7

From: Cell surface heparan sulfate proteoglycans contribute to intracellular lipid accumulation in adipocytes

Figure 7

Reduction in intracellular lipid accumulation in adipocytes by heparan sulfate inhibitors is concentration-dependent. 4-MUmb (solid bars) or pNP-Xyl (hatched bars) was added to 3T3-L1 cells at the indicated concentrations concurrently with differentiation reagents as in Fig. 5. Cells were then fixed with 10% formaldehyde, stained with 0.1% Oil Red O, and cell-associated lipophilic dye was extracted with 0.1 N HCl and quantitated by spectrophotometry. Data shown represents an average of three experiments.

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