Effects of the calcium deficient diet for 2 1/2 months on the development of lesions and calcification in sibling rabbits. The cross sections of the middle section of aortas were stained with H&E (Panels A and B) and the proximal section with AR (Panel C and D). Panels A and C: Cross-sections obtained from a sibling fed the atherogenic diet with normal levels of Ca. Panels B and D: The sections from a sibling fed the atherogenic diet with calcium deficiency. The semi-quantification for intimal thickening and calcification was described in Fig. 1 legend. Panel E provides the paired Student's t-test for the significance of the differences in these ratios between the two respective groups each with 11 siblings. Note that the graphic bar representing calcification for normal dietary Ca groups was not seen since there was no calcification in this group during 2 1/2 months of cholesterol intervention.