Figure 4

³H-sulfatide uptake in isolated pancreatic islets. The autoradiography plates are showing the uptake of ³H-sulfatide isoforms (C12:0, C16:0 and C24:0) in isolated rat islets of Langerhans. Isolation and culturing of islets, lipid isolation, thin-layer chromatography and autoradiography are described under "Methods". Aliquots of the saponified "sulfatide fractions", corresponding to 4 000–20 000 dpm (islets cultured with C12:0 and C24:0 sulfatide) or 40 000–100 000 dpm (islets cultured with C16:0 sulfatide) were applied to the plates (one representative plate is shown). Lane 1, ³H-sphingomyelin, ³H-sulfatide, ³H-galactosylceramide and ³H-ceramide standards. A. Lane 2, ³H-C12:0 sulfatide standard; uptake of ³H-C12:0 sulfatide in rat islets after 6 hours (lane 3) and 24 hours (lane 4). B. Lane 2, ³H-C16:0 sulfatide standard; uptake of ³H-C16:0 sulfatide in rat islets after 6 hours (lane 3) and 24 hours (lane 4). C. Lane 2, ³H-C24:0 sulfatide standard; uptake of ³H-C24:0 sulfatide in rat islets after 6 hours (lane 3) and 24 hours (lane 4).