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Figure 2 | Lipids in Health and Disease

Figure 2

From: Synthesis of the oxysterol, 24(S), 25-epoxycholesterol, parallels cholesterol production and may protect against cellular accumulation of newly-synthesized cholesterol

Figure 2

Endogenously-produced 24,25EC correlates with cholesterol synthesis in different CHO cell-lines. (A) Various Chinese Hamster Ovary cell-types with different abilities to synthesise cholesterol were incubated in the presence of [1-14C]-acetate for 24 h. KEY: 1. CHO-7; 2. SRD-1; 3. M19; 4. SRD-13A; 5. 13A/pSCAP. For more details about these cell-lines, see [25]. Neutral lipid extracts were separated by thin-layer chromatography and bands corresponding to authentic cholesterol and 24,25EC were visualized by phosphorimager and quantified by densitometry. Equations for the lines of best fit (B) y = 0.97x + 0.39; R2 = 0.89; P < 0.03. Values are means from 3 separate experiments set relative to CHO-7 cells which was assigned an arbitrary value of 1.

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Lipids in Health and Disease

ISSN: 1476-511X