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Table 5 Fatty Acid Composition of Rat White Gastrocnemius After Dietary Supplementation with 22:6n-3 and 22:5n-6*

From: Docosahexaenoic acid and n-6 docosapentaenoic acid supplementation alter rat skeletal muscle fatty acid composition

  

Artificially reared

 

Dam reared (n = 7)

AR-LA (n = 7)

AR-DPA (n = 6)

AR-DHA+DPA (n = 8)

AR-DHA (n = 7)

Fatty acids

(weight % of total fatty acids)

12:0

5.0 ± 1.8a

1.50 ± 0.85b

1.98 ± 0.54b

2.08 ± 0.24b

1.93 ± 0.68b

14:0

4.3 ± 1.1a

2.11 ± 0.69b

2.68 ± 0.48b

2.73 ± 0.21b

2.49 ± 0.45b

16:0 DMA

1.35 ± 0.41

1.22 ± 0.55

0.95 ± 0.39

1.03 ± 0.25

1.17 ± 0.32

16:0

24.7 ± 1.0

26.0 ± 1.9

25.7 ± 0.4

25.9 ± 1.0

25.0 ± 1.2

18:0 DMA

0.71 ± 0.26a

0.41 ± 0.17b

0.35 ± 0.21b

0.36 ± 0.10b

0.43 ± 0.17ab

18:0

7.2 ± 0.9a

6.9 ± 1.7ab

5.7 ± 1.6ab

5.2 ± 0.8b

5.7 ± 1.5ab

20:0

0.05 ± 0.01a

0.04 ± 0.01ab

0.03 ± 0.01b

0.03 ± 0.01b

0.04 ± 0.01ab

22:0

0.05 ± 0.01a

0.04 ± 0.01ab

0.03 ± 0.01b

0.03 ± 0.01b

0.03 ± 0.01ab

24:0

0.10 ± 0.02a

0.06 ± 0.02b

0.05 ± 0.02b

0.06 ± 0.02b

0.08 ± 0.03ab

Total saturates

43.4 ± 1.3a

38.4 ± 2.5b

37.5 ± 1.0b

37.4 ± 0.9b

36.8 ± 1.3b

16:1 n-7

6.3 ± 1.4

5.4 ± 1.6

7.1 ± 2.0

7.0 ± 1.5

6.2 ± 1.9

18:1 DMA

0.18 ± 0.07

0.26 ± 0.08

0.21 ± 0.12

0.27 ± 0.08

0.30 ± 0.10

18:1 n-9

14.7 ± 2.8a

21.8 ± 7.3ab

27.0 ± 4.4b

27.6 ± 2.5b

25.9 ± 5.0b

18:1 n-7

3.60 ± 0.19a

3.78 ± 0.41ab

3.86 ± 0.25ab

4.04 ± 0.34ab

4.20 ± 0.34b

20:1 n-9

0.08 ± 0.02a

0.14 ± 0.05b

0.15 ± 0.05b

0.16 ± 0.02b

0.14 ± 0.03b

24:1 n-9

0.09 ± 0.04

0.10 ± 0.04

0.08 ± 0.03

0.10 ± 0.04

0.12 ± 0.05

Total monounsaturates

24.9 ± 4.0a

31.5 ± 8.8ab

38.4 ± 6.3b

39.1 ± 3.6b

36.9 ± 6.1b

18:2 n-6

11.6 ± 1.0a

10.0 ± 0.9b

8.1 ± 1.2c

7.7 ± 0.7c

8.3 ± 1.0c

18:3 n-6

0.033 ± 0.005ab

0.037 ± 0.003a

0.029 ± 0.004bc

0.025 ± 0.003c

0.027 ± 0.003c

20:2 n-6

0.05 ± 0.01a

0.04 ± 0.01b

0.03 ± 0.01b

0.03 ± 0.01b

0.03 ± 0.01b

20:3 n-6

0.34 ± 0.08

0.44 ± 0.16

0.32 ± 0.09

0.29 ± 0.07

0.31 ± 0.08

20:4 n-6

6.4 ± 2.0ab

9.8 ± 3.9a

6.2 ± 2.2ab

5.4 ± 1.3b

6.2 ± 2.2ab

22:4 n-6

0.27 ± 0.08a

0.81 ± 0.28b

0.42 ± 0.12a

0.24 ± 0.05a

0.25 ± 0.07a

22:5 n-6

0.37 ± 0.16a

4.5 ± 1.6b

5.0 ± 1.8b

1.17 ± 0.29a

0.81 ± 0.32a

Total n-6 PUFA

19.1 ± 2.7a

25.7 ± 6.7b

20.1 ± 5.1ab

14.9 ± 2.1a

15.9 ± 3.0a

18:3 n-3

0.61 ± 0.17a

0.02 ± 0.01b

0.018 ± 0.003b

0.03 ± 0.02b

0.02 ± 0.01b

20:5 n-3

0.10 ± 0.02

-

-

0.02 ± 0.01

0.02 ± 0.01

22:5 n-3

0.86 ± 0.22a

0.09 ± 0.04b

0.06 ± 0.02b

0.09 ± 0.02b

0.13 ± 0.05b

22:6 n-3

7.7 ± 1.8a

0.58 ± 0.25b

0.66 ± 0.40b

5.4 ± 1.3c

7.0 ± 2.1ac

Total n-3 PUFA

9.3 ± 1.9a

0.69 ± 0.28b

0.74 ± 0.41b

5.6 ± 1.3c

7.1 ± 2.1ac

Total PUFA

28.3 ± 4.5

26.4 ± 6.9

20.8 ± 5.4

20.5 ± 3.2

23.1 ± 5.0

Total HUFA

16.0 ± 4.3

16.2 ± 6.1

12.6 ± 4.4

12.6 ± 2.7

14.7 ± 4.6

% of n-3 HUFA in HUFA

54.2 ± 2.0a

4.1 ± 0.4b

5.7 ± 2.2b

43.8 ± 3.1c

48.6 ± 3.6d

Total FA (mg/g)

15.9 ± 3.5

16.3 ± 9.1

21.2 ± 7.6

21.1 ± 4.6

17.9 ± 6.3

  1. *Values are mean ± SD. Values in a row not sharing a roman superscript are significantly different at P < 0.05 by Tukey's honestly significantly difference test after a significant F-value (P < 0.05) by one-way ANOVA. AR-LA, 18:2n-6 artificial reared diet; AR-DPAn-6, 22:5n-6 artificial reared diet; AR-DHA+DPAn-6, 22:6n-3 + 22:5n-6 artificial reared diet; AR-DHA, 22:6n-3 artificial reared diet; DMA, dimethyl acetal; PUFA, polyunsaturated fatty acids; HUFA, highly unsaturated fatty acids (≥ 20 carbons, ≥ 3 carbon-carbon double bonds)