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Table 5 Fatty Acid Composition of Rat White Gastrocnemius After Dietary Supplementation with 22:6n-3 and 22:5n-6*

From: Docosahexaenoic acid and n-6 docosapentaenoic acid supplementation alter rat skeletal muscle fatty acid composition

   Artificially reared
  Dam reared (n = 7) AR-LA (n = 7) AR-DPA (n = 6) AR-DHA+DPA (n = 8) AR-DHA (n = 7)
Fatty acids (weight % of total fatty acids)
12:0 5.0 ± 1.8a 1.50 ± 0.85b 1.98 ± 0.54b 2.08 ± 0.24b 1.93 ± 0.68b
14:0 4.3 ± 1.1a 2.11 ± 0.69b 2.68 ± 0.48b 2.73 ± 0.21b 2.49 ± 0.45b
16:0 DMA 1.35 ± 0.41 1.22 ± 0.55 0.95 ± 0.39 1.03 ± 0.25 1.17 ± 0.32
16:0 24.7 ± 1.0 26.0 ± 1.9 25.7 ± 0.4 25.9 ± 1.0 25.0 ± 1.2
18:0 DMA 0.71 ± 0.26a 0.41 ± 0.17b 0.35 ± 0.21b 0.36 ± 0.10b 0.43 ± 0.17ab
18:0 7.2 ± 0.9a 6.9 ± 1.7ab 5.7 ± 1.6ab 5.2 ± 0.8b 5.7 ± 1.5ab
20:0 0.05 ± 0.01a 0.04 ± 0.01ab 0.03 ± 0.01b 0.03 ± 0.01b 0.04 ± 0.01ab
22:0 0.05 ± 0.01a 0.04 ± 0.01ab 0.03 ± 0.01b 0.03 ± 0.01b 0.03 ± 0.01ab
24:0 0.10 ± 0.02a 0.06 ± 0.02b 0.05 ± 0.02b 0.06 ± 0.02b 0.08 ± 0.03ab
Total saturates 43.4 ± 1.3a 38.4 ± 2.5b 37.5 ± 1.0b 37.4 ± 0.9b 36.8 ± 1.3b
16:1 n-7 6.3 ± 1.4 5.4 ± 1.6 7.1 ± 2.0 7.0 ± 1.5 6.2 ± 1.9
18:1 DMA 0.18 ± 0.07 0.26 ± 0.08 0.21 ± 0.12 0.27 ± 0.08 0.30 ± 0.10
18:1 n-9 14.7 ± 2.8a 21.8 ± 7.3ab 27.0 ± 4.4b 27.6 ± 2.5b 25.9 ± 5.0b
18:1 n-7 3.60 ± 0.19a 3.78 ± 0.41ab 3.86 ± 0.25ab 4.04 ± 0.34ab 4.20 ± 0.34b
20:1 n-9 0.08 ± 0.02a 0.14 ± 0.05b 0.15 ± 0.05b 0.16 ± 0.02b 0.14 ± 0.03b
24:1 n-9 0.09 ± 0.04 0.10 ± 0.04 0.08 ± 0.03 0.10 ± 0.04 0.12 ± 0.05
Total monounsaturates 24.9 ± 4.0a 31.5 ± 8.8ab 38.4 ± 6.3b 39.1 ± 3.6b 36.9 ± 6.1b
18:2 n-6 11.6 ± 1.0a 10.0 ± 0.9b 8.1 ± 1.2c 7.7 ± 0.7c 8.3 ± 1.0c
18:3 n-6 0.033 ± 0.005ab 0.037 ± 0.003a 0.029 ± 0.004bc 0.025 ± 0.003c 0.027 ± 0.003c
20:2 n-6 0.05 ± 0.01a 0.04 ± 0.01b 0.03 ± 0.01b 0.03 ± 0.01b 0.03 ± 0.01b
20:3 n-6 0.34 ± 0.08 0.44 ± 0.16 0.32 ± 0.09 0.29 ± 0.07 0.31 ± 0.08
20:4 n-6 6.4 ± 2.0ab 9.8 ± 3.9a 6.2 ± 2.2ab 5.4 ± 1.3b 6.2 ± 2.2ab
22:4 n-6 0.27 ± 0.08a 0.81 ± 0.28b 0.42 ± 0.12a 0.24 ± 0.05a 0.25 ± 0.07a
22:5 n-6 0.37 ± 0.16a 4.5 ± 1.6b 5.0 ± 1.8b 1.17 ± 0.29a 0.81 ± 0.32a
Total n-6 PUFA 19.1 ± 2.7a 25.7 ± 6.7b 20.1 ± 5.1ab 14.9 ± 2.1a 15.9 ± 3.0a
18:3 n-3 0.61 ± 0.17a 0.02 ± 0.01b 0.018 ± 0.003b 0.03 ± 0.02b 0.02 ± 0.01b
20:5 n-3 0.10 ± 0.02 - - 0.02 ± 0.01 0.02 ± 0.01
22:5 n-3 0.86 ± 0.22a 0.09 ± 0.04b 0.06 ± 0.02b 0.09 ± 0.02b 0.13 ± 0.05b
22:6 n-3 7.7 ± 1.8a 0.58 ± 0.25b 0.66 ± 0.40b 5.4 ± 1.3c 7.0 ± 2.1ac
Total n-3 PUFA 9.3 ± 1.9a 0.69 ± 0.28b 0.74 ± 0.41b 5.6 ± 1.3c 7.1 ± 2.1ac
Total PUFA 28.3 ± 4.5 26.4 ± 6.9 20.8 ± 5.4 20.5 ± 3.2 23.1 ± 5.0
Total HUFA 16.0 ± 4.3 16.2 ± 6.1 12.6 ± 4.4 12.6 ± 2.7 14.7 ± 4.6
% of n-3 HUFA in HUFA 54.2 ± 2.0a 4.1 ± 0.4b 5.7 ± 2.2b 43.8 ± 3.1c 48.6 ± 3.6d
Total FA (mg/g) 15.9 ± 3.5 16.3 ± 9.1 21.2 ± 7.6 21.1 ± 4.6 17.9 ± 6.3
  1. *Values are mean ± SD. Values in a row not sharing a roman superscript are significantly different at P < 0.05 by Tukey's honestly significantly difference test after a significant F-value (P < 0.05) by one-way ANOVA. AR-LA, 18:2n-6 artificial reared diet; AR-DPAn-6, 22:5n-6 artificial reared diet; AR-DHA+DPAn-6, 22:6n-3 + 22:5n-6 artificial reared diet; AR-DHA, 22:6n-3 artificial reared diet; DMA, dimethyl acetal; PUFA, polyunsaturated fatty acids; HUFA, highly unsaturated fatty acids (≥ 20 carbons, ≥ 3 carbon-carbon double bonds)