Skip to main content

Table 1 List of identified proteins related to calcification in calcifying vesicles from tadem spectrometry. Data were analyzed using a Biowors 3.2 (ThermoFinnigan) program.

From: Induction of calcification by serum depletion in cell culture: a model for focal calcification in aortas related to atherosclerosis

Proteins

Peptide Sequence

Calpactin I Heavy chain

SNAQRQDIAFAYQR (2, 4.50, 0.32)

LSLEGDHSTPPSAYGSVK (2, 4.00, 0.40)

RAEDGSVIDYELIDQDAR (2, 3.69, 0.29)

GVDEVTIVNILTNR (1, 2.83, 0.29)

Calreticulin or CRP55

FYALSAR (1, 2.00, 0.44)

FKPFSNKGQPLVVQFTVK (2, 3.75, 0.64)

EPVVYFKEQFLDGDGWTER.G (2, 10.2, 0.54)

Integrin beta 1

SKGTAEKLQPEDITQIQPQQLVLQLR (1, 2.02, 0.36)

GTAEKLQPEDITQIQPQQLVLQLR (2, 5.42, 0.54)

LQPEDITQIQPQQLVLQLR (2, 5.42, 0.54)

SGEPQTFTLK (2, 2.02, 0.36)

Fibrillin

IKGTQC#EDINEC#EVFPGVC#K (2, 3.00, 0.36)

IKGTQC#EDIDEC#EVFPGVCKNGLCVNTR (3, 4.58, 0.35)

ATPase β subunit

VALTGLTVAEYFRDEEGQDVLLFIDNIFR (3, 2.70, 0.27)

AIAELGIYPAVDPLDSTSR (2, 4.76, 0.12)

ATP synthase

ITSTKEGSITSIQAIYVPADDLTDPAPATTFAHLDATTVLSR (3, 5.69, 0.17)

VLDSGAPIKIPVGPETLGR (2, 3.81, 0.65)

ILQDYKSLQDIIAILGMDELSEEDKLTVSR (3, 3.78, 0.55)

ILGADTSVDLEETGRVLSIGDGIAR (2, 3.26, 0.19)

TGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSK (2, 5.13, 0.47)

AIAELGIYPAVDPLDSTSR (1, 3.0, 0.46)

  1. The following set of criteria for high confidence were used: minimum cross-correlation score of (Xcorr) of 1.9, 2.5 and 3.5 for 1, 2 and 3 charges ions respectively, a delta correlation score (Δcorr) greater than 0.1, and a minimum of 2. These parameters are presented in the parentheses in sequence of charges, Xcorr, and Δcorr. Database used was obtained from the NCBI repository. C# indicates a carboxymethylcisteine.
Back to article page

Lipids in Health and Disease

ISSN: 1476-511X