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Figure 6 | Lipids in Health and Disease

Figure 6

From: The omega-3 fatty acid, eicosapentaenoic acid (EPA), prevents the damaging effects of tumour necrosis factor (TNF)-alpha during murine skeletal muscle cell differentiation

Figure 6

EPA inhibits the TNF-α-induced caspase-8 activity associated with apoptosis. The nature of the pro-apoptotic effect of TNF-α on C2C12 myotube formation and its prevention by EPA were examined after 24 or 48 hours in DM by evaluating caspase-8 activation (measured in relative light units, RLU) using the Caspase Glo-8 commercial assay. EPA was added together with TNF-α as a co-treatment (EPA+TNF) or, alternatively EPA was administered alone for a 2 hour pre-treatment after which it was withdrawn and replaced by TNF-α alone in DM (pEPA+TNF). Data are expressed as means ± standard error of mean (SEM) from 3 independent experiments. (*p < 0.05 v. respective control; #NS v. respective control).

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Lipids in Health and Disease

ISSN: 1476-511X