Table 5 Primers for Real-time RT-PCR
Gene | Primer orientation | Neucleotide sequence | Amplicon (bp) | NCBI Accetion number |
|---|---|---|---|---|
Beta-actin | forward | 5-CCCTAGACTTCGAGCAAGAG-3 | 155 | NM_031144.2 |
| Â | reverse | 5-GGATTCCATACCCAGGAAGG-3 | Â | Â |
EGFR | forward | 5-CCCACAGCAAGGCTTCTTCA-3 | 119 | NM_031507.1 |
| Â | reverse | 5-CACGGCAGCTCCCATTTCTA-3 | Â | Â |
FABP7 | forward | 5-ATGGAGACAAGCTCATTCATGTG-3 | 134 | NM_030832 |
| Â | reverse | 5-TGCCTTTTCATAACAGCGAACA-3 | Â | Â |
GAPDH | forward | 5-GTGCTGAGTATGTCGTGGAGTCTA-3 | 144 | NM_017008.3 |
| Â | reverse | 5-TCTCGTGGTTCACACCCATCAC-3 | Â | Â |
PPARγ | forward | 5-CTGACCCAATGGTTGCTGATTAC-3 | 80 | NM_013124.2 |
| Â | reverse | 5-GGACGCAGGCTCTACTTTGATC-3 | Â | Â |
RXRα | forward | 5-GAGGACATGCCTGTAGAGAAGATT-3 | 126 | NM_012805.2 |
| Â | reverse | 5-ACAGATGTTGGTAACAGGGTCATT-3 | Â | Â |