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Table 5 Primers for Real-time RT-PCR

From: The influence of feeding linoleic, gamma-linolenic and docosahexaenoic acid rich oils on rat brain tumor fatty acids composition and fatty acid binding protein 7 mRNA expression

Gene Primer orientation Neucleotide sequence Amplicon (bp) NCBI Accetion number
Beta-actin forward 5-CCCTAGACTTCGAGCAAGAG-3 155 NM_031144.2
  reverse 5-GGATTCCATACCCAGGAAGG-3   
EGFR forward 5-CCCACAGCAAGGCTTCTTCA-3 119 NM_031507.1
  reverse 5-CACGGCAGCTCCCATTTCTA-3   
FABP7 forward 5-ATGGAGACAAGCTCATTCATGTG-3 134 NM_030832
  reverse 5-TGCCTTTTCATAACAGCGAACA-3   
GAPDH forward 5-GTGCTGAGTATGTCGTGGAGTCTA-3 144 NM_017008.3
  reverse 5-TCTCGTGGTTCACACCCATCAC-3   
PPARγ forward 5-CTGACCCAATGGTTGCTGATTAC-3 80 NM_013124.2
  reverse 5-GGACGCAGGCTCTACTTTGATC-3   
RXRα forward 5-GAGGACATGCCTGTAGAGAAGATT-3 126 NM_012805.2
  reverse 5-ACAGATGTTGGTAACAGGGTCATT-3