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Figure 3 | Lipids in Health and Disease

Figure 3

From: Enhanced production of nitric oxide, reactive oxygen species, and pro-inflammatory cytokines in very long chain saturated fatty acid-accumulated macrophages

Figure 3

Intracellular ROS productions in macrophages. Cells were incubated with 10 μM of DCFH-DA for 15 min, and then stimulated with 0.5 μg/ml of PMA for 20 min at 37°C. Fluorescence of DCF was measured by a flow cytometer as described in Methods. Values are mean-fold increase of relative DCF fluorescence intensity compared with non-stimulated control and expressed as mean ± SD, n = 6. * P < 0.05 for comparison with wild-type.

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Lipids in Health and Disease

ISSN: 1476-511X