Skip to main content
Figure 5 | Lipids in Health and Disease

Figure 5

From: Arachidonic acid increases matrix metalloproteinase 9 secretion and expression in human monocytic MonoMac 6 cells

Figure 5

Inhibition of arachidonic acid (AA) and PMA induced stimulation of MMP-9 secretion by LY294002. Dose- and time-dependent stimulation of MMP-9 secretion by arachidonic acid. MonoMac 6 cells (0.8 × 106 cells/ml in X-Vivo 15) were treated with various concentrations of LY294002 in DMSO for 30 min. Then 10 μM arachidonic acid (5A) or 1.3 nM PMA (5B) was added and the incubation was continued for 24 h. The media were analysed for the presence of MMP-9 using zymography and image analysis. Results (mean ± SEM) for 4 to 5 independent experiments are shown. All concentrations of LY294002 decreased the AA or PMA induced secretion of MMP-9 statistically significantly.

Back to article page

Lipids in Health and Disease

ISSN: 1476-511X