Figure 1From: The solubilisation of boar sperm membranes by different detergents - a microscopic, MALDI-TOF MS, 31P NMR and PAGE study on membrane lysis, extraction efficiency, lipid and protein compositionAmounts of phosphate (as the measure of phospholipids) and cholesterol determined by biochemical assays in the organic extracts of detergent-treated boar spermatozoa. From these data, the ratio between cholesterol and phosphate (C/P) was also calculated. Sperm cells were incubated with detergents for 30 min at 4°C and lipids were extracted and determined as described in the Materials and Methods section. The mean ± SD of two different extractions per sample is shown. Please note that there are only slight differences between the individual detergents.Back to article page