Skip to main content

Table 1 Percentage (mean ± SD) of boar sperm cells with intact, detaching but still adherent, or completely detached head membranes after incubation with detergents for 30 min at 4°C or 38°C

From: The solubilisation of boar sperm membranes by different detergents - a microscopic, MALDI-TOF MS, 31P NMR and PAGE study on membrane lysis, extraction efficiency, lipid and protein composition

 

intact membranes

detaching membranes

detached membranes

Detergent

4°C

38°C

4°C

38°C

4°C

38°C

w/o

91,2 ± 8,5

92,7 ± 5,1

8,3 ± 8,6

6,1 ± 3,7

0,4 ± 0,7

1,2 ± 2,3

Pluronic F-127

94,1 ± 4,5

95,3 ± 4,0

5,6 ± 4,7

4,1 ± 3,8

0,3 ± 0,5

1,0 ± 1,2

Sodium cholate

0,3 ± 0,8

0,1 ± 0,4

96,0 ± 3,4

97,1 ± 2,3

3,7 ± 3,1

2,7 ± 2,2

CHAPS

0,3 ± 0,8

0,0 ± 0,0

96,4 ± 5,7

94,7 ± 3,5

3,3 ± 5,8

5,3 ± 3,5

Tween 20

43,1 ± 25,1**

0,0 ± 0,0**

56,0 ± 24,7**

98,0 ± 1,7**

0,9 ± 0,9

2,0 ± 1,7

Triton X-100

0,1 ± 0,3

0,6 ± 0,9

97,2 ± 2,5

96,7 ± 2,0

2,7 ± 2,6

2,8 ± 1,9

Brij 96V

0,1 ± 0,3

0,3 ± 0,5

98,8 ± 1,9*

91,8 ± 7,6*

1,1 ± 1,6*

7,9 ± 7,7*

  1. * significant difference in dependence of temperature (student t-test for paired samples, p ≤ 0,05, n = 9)
  2. ** significant difference in dependence of temperature (student t-test for paired samples, p ≤ 0,01, n = 9)
  3. Sperm cells of ejaculates from 9 different boars were treated and fixed as described in the "Materials and Methods" section. 200 cells per sample were counted under phase contrast optics (magnification of 1000).