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Table 1 Percentage (mean ± SD) of boar sperm cells with intact, detaching but still adherent, or completely detached head membranes after incubation with detergents for 30 min at 4°C or 38°C

From: The solubilisation of boar sperm membranes by different detergents - a microscopic, MALDI-TOF MS, 31P NMR and PAGE study on membrane lysis, extraction efficiency, lipid and protein composition

  intact membranes detaching membranes detached membranes
Detergent 4°C 38°C 4°C 38°C 4°C 38°C
w/o 91,2 ± 8,5 92,7 ± 5,1 8,3 ± 8,6 6,1 ± 3,7 0,4 ± 0,7 1,2 ± 2,3
Pluronic F-127 94,1 ± 4,5 95,3 ± 4,0 5,6 ± 4,7 4,1 ± 3,8 0,3 ± 0,5 1,0 ± 1,2
Sodium cholate 0,3 ± 0,8 0,1 ± 0,4 96,0 ± 3,4 97,1 ± 2,3 3,7 ± 3,1 2,7 ± 2,2
CHAPS 0,3 ± 0,8 0,0 ± 0,0 96,4 ± 5,7 94,7 ± 3,5 3,3 ± 5,8 5,3 ± 3,5
Tween 20 43,1 ± 25,1** 0,0 ± 0,0** 56,0 ± 24,7** 98,0 ± 1,7** 0,9 ± 0,9 2,0 ± 1,7
Triton X-100 0,1 ± 0,3 0,6 ± 0,9 97,2 ± 2,5 96,7 ± 2,0 2,7 ± 2,6 2,8 ± 1,9
Brij 96V 0,1 ± 0,3 0,3 ± 0,5 98,8 ± 1,9* 91,8 ± 7,6* 1,1 ± 1,6* 7,9 ± 7,7*
  1. * significant difference in dependence of temperature (student t-test for paired samples, p ≤ 0,05, n = 9)
  2. ** significant difference in dependence of temperature (student t-test for paired samples, p ≤ 0,01, n = 9)
  3. Sperm cells of ejaculates from 9 different boars were treated and fixed as described in the "Materials and Methods" section. 200 cells per sample were counted under phase contrast optics (magnification of 1000).