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Figure 3 | Lipids in Health and Disease

Figure 3

From: Gamma-linolenic acid inhibits both tumour cell cycle progression and angiogenesis in the orthotopic C6 glioma model through changes in VEGF, Flt1, ERK1/2, MMP2, cyclin D1, pRb, p53 and p27 protein expression

Figure 3

Effect of 14-day osmotic pump infusion of 5 mM gamma-linolenic acid (GLA) or control cerebrospinal fluid (CON = CSF) on cell cycle control in the C6 glioma in vivo. (A) Western blot of pRb protein expression, statistical significance, * = p < 0.009. (B) Western blot of E2F1 protein expression. (C) Western blot of cyclin D1 protein expression, statistical significance, * = p < 0.008. (D) Western blot of p21 protein expression. (E) Western blot of p27 protein expression, statistical significance, * = p < 0.005. (F) Western blot of p53 protein expression, statistical significance, * = p < 0.026. For all Western blots GAPDH was used as an internal control and is presented below the protein bands of interest in A-F, (n = 11), Lanes 1–5 CON, 6–11 GLA. (G) Bromodeoxyuridine incorporation in vivo, representative image of labelled cells (n = 9), statistical significance, * = p < 0.015. (H) Schematic presentation of changes found in GLA-treated tumours in comparison with control tumours and possible interactions among proteins based on current literature.

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Lipids in Health and Disease

ISSN: 1476-511X