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Figure 2 | Lipids in Health and Disease

Figure 2

From: Ovalbumin-induced plasma interleukin-4 levels are reduced in ceramide kinase-deficient DO11.10 RAG1-/- mice

Figure 2

IL-4 release in OVA challenged isolated splenocytes. Splenocytes from DO11.10 RAG1-/- and from Cerk-/- DO11.10 RAG1-/- mice were isolated following ovalbumin challenge in vivo as described in the Experimental section. Cultured splenocytes were then incubated with 10 μg/ml OVA323-339 peptide or with saline (control) for 3.5 hours and IL-4 levels were measured in cell culture supernatant. The data shown represent the mean ± SD of measurements obtained from 8 animals (OVA-treated) and 2 animals (controls). In this experiment it was not possible to match the age of the animal population optimally: DO11.10 RAG1-/- were 8 weeks of age at start of the experiment whereas Cerk-/- DO11.10 RAG1-/- mice were between 11 and 14 weeks of age.

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Lipids in Health and Disease

ISSN: 1476-511X