Figure 3
From: Reduced levels of SCD1 accentuate palmitate-induced stress in insulin-producing β-cells
Levels of markers of ER stress and apoptosis in SCD1 KD and mock-transfected MIN6 cells after palmitate exposure. MIN6 cells were transfected with siRNA targeted towards SCD1 (black square) or with nonsense siRNA (mock; white square) and cultured for the indicated time periods in the presence of 0.25 mM palmitate. After culture, protein levels of p-eIF2α (panel A), CHOP (panel B), and cleaved caspase-3 (panel C) were measured. Results are means ± SEM from 3-6 individual experiments. *P < 0.05 denotes effect of SCD1 KD compared to mock-transfected cells at indicated time point and #P < 0.05 denotes effect of palmitate compared to time 0.