Figure 3
Effects of δ-tocotrienol (concentration 10 μM - 640 μM) on proteasomal active sites, chymotrypsin-like, trypsin-like and post-glutamase, after 60 min treatment of RAW 264 .7 whole cells. Proteasome-Glo (Promega) chymotrypsin-like, trypsin-like, and post-glutamase cell based assays were carried out. 10,000 cells were plated per well in 100 μL of media in a 96-well white colored plate. Cells were allowed to adhere to plates for 2 h prior to testing. At the start of the test, media or 0.4% DMSO + media (used as control = CD), or δ-tocotrienol (concentrations 10 μM - 640 μM) dissolved in 0.4% DMSO were added to each well for a duration of 1 h. The mixtures in the plates were assayed according to Promega Protocol. Plates were read with a "Promega Luminometer" according to the directions of manufacturer, which give relative luminescence units (RLV) values.