Figure 1
From: Curcumin inhibits cholesterol uptake in Caco-2 cells by down-regulation of NPC1L1 expression
Cholesterol uptake by Caco-2 cells is mediated by NPC1L1. The cells were cultured to 100% confluence and pretreated with ezetimibe at different concentrations for 2 h. The cells were then incubated with radioactive micellar cholesterol for 2 h. The cells were then lysed and the uptake of radioactive cholesterol was quantified by liquid scintillation. Results are mean ± SEM of duplicate determinations in three separate experiments. * P < 0.05, ** P < 0.01.