Figure 4
From: Human apoA-I increases macrophage foam cell derived PLTP activity without affecting the PLTP mass
The capacity of lipidated apoA-I and other apolipoproteins to induce PLTP activity. Panel A, PLTP was incubated for 2 hours at room temperature in the presence of 10 μg/ml of lipid free apoA-I or lipidated with increasing amounts of phosphatidylcholine (PC) and cholesterol (CHOL) and 10 μg/ml HDL (representing a highly lipidated form of apoA-I) and assayed for the phospholipid transfer activity. Panel B, PLTP was incubated for 2 hours at room temperature in the presence of 10 μg/ml of purified rabbit imunoglobulins (IgG), bovine serum albumin (BSA), apoA-I, apoA-II, apoA-IV, apoE and HDL and analyzed for phospholipid transfer activity. Figures are representative for three independent experiments. Bars and error bars represent mean ± SD calculated from triplicates. * P < 0.05, **P < 0.01