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Figure 3 | Lipids in Health and Disease

Figure 3

From: Fish oil supplementation reverses the effect of cholesterol on apoptotic gene expression in smooth muscle cells

Figure 3

25-hidroxycholesterol effects on p53 expression in the cell culture model. p53 mRNA quantification in SMC cultures (SMC-C, SMC-Ch, SMC-Ch-FO) and SMC cultures treated with 20 μg/mL 25-hydroxycholesterol for 24 h. mRNA levels were quantified by real-time semiquantitative reverse-transcription PCR. Results are shown as relative expression ratio of p53 in SMC cultures with respect to control culture and expressed in comparison to reference gene β-actin. *P < 0.05, ** P < 0.01, *** P < 0.001 vs. SMC-C, +P < 0.05, ++P < 0.01, +++ P < 0.001 vs. SMC-C treated with 20 μg/mL 25-hydroxycholesterol.

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Lipids in Health and Disease

ISSN: 1476-511X