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Figure 4 | Lipids in Health and Disease

Figure 4

From: High-salt in addition to high-fat diet may enhance inflammation and fibrosis in liver steatosis induced by oxidative stress and dyslipidemia in mice

Figure 4

Hepatic ROS accumulation in the liver of each treated group of mice. a, The NADPH oxidase activity (n = 6-11/group). b, Representative histological findings with immunohistochemical staining for 4-hydroxynonenal (4-HNE: original magnification, X400). c, 4-HNE protein in the whole liver (n = 4 for each group). d, Oxygen radical formation in Kupffer cells after H2O2 preconditioning. Formazan depositions are indicated by blue arrowheads. (nuclear red staining: original magnification, X1000). e, Histological analysis of anti-oxidant enzymes activity. Representative SOD-1 immunohistochemical stainning (original magnification, X400). f, Histological analysis of anti-oxidant enzymes activity. Representative catalase immunohistochemical stainning (original magnification, X400). g, Histological analysis of anti-oxidants. Representative glutathione immunohistochemical stainning (original magnification, X400). See abbreviations in the legends of Figure 1. Values are means ± SEM. *P < 0.001 compared with mice fed ND. **P < 0.01 compared with mice fed ND.

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Lipids in Health and Disease

ISSN: 1476-511X