Induction of omega 6 inflammatory pathway by sodium metabisulfite in rat liver and its attenuation by ghrelin

Table 1 Analysis of polyunsaturated fatty acids in liver tissue

Parameter	Control (*n = 8*)	Na₂S₂O₅(*n = 10*)	Ghrelin (*n = 10*)	Na₂S₂O₅ + Ghrelin (*n = 10*)
DGLA (C20:3n6)	3.73 ± 1.26	9.44 ± 2.32^a	3.65 ± 1.15	5.98 ± 2.52^b
AA (C20:4n6)	17.73 ± 7.74	90.44 ± 32.63^c,d	29.43 ± 9.09	54.89 ± 10.57^b,e
EPA (C20:5n3)	0.85 ± 0.50	1.11 ± 0.47	1.12 ± 0.38	1.13 ± 0.60
DHA (C22:6n3)	10.14 ± 3.29	11.96 ± 7.45	11.42 ± 4.46	9.74 ± 2.56
AA/DHA	3.35 ± 1.17	9.33 ± 3.02^f	3.05 ± 1.02	4.01 ± 0.71
AA/EPA	27.66 ± 14.21	87.66 ± 37.24^f	26.60 ± 7.67	34.36 ± 7.64

Values are expressed as mg fatty acid/g tissue protein. Data are reported as mean ± SD. Na₂S₂O₅, sodium metabisulfite; DGLA, Dihomo-gamma-linolenic acid; AA, Arachidonic acid; EPA, Eicosapentaenoic acid; DHA, Docosahexaenoic acid. Statistical analysis was performed by one way analysis of variance with all pairwise multiple comparisons via Tukey test or Kruskal-Wallis One Way Analysis of Variance on Ranks with all pairwise multiple comparison procedures by Dunn's Method.
^ap ≤ 0.001 Na₂S₂O₅ vs. control, ghrelin, Na₂S₂O₅ + ghrelin.
^bp < 0.05 Na₂S₂O₅ + ghrelin vs. Ghrelin.
^cp < 0.001 Na₂S₂O₅ vs. control, ghrelin.
^dp = 0.002 Na₂S₂O₅ vs. Na₂S₂O₅ + ghrelin.
^ep = 0.002 Na₂S₂O₅ + ghrelin vs. control.
^fp < 0.05 Na₂S₂O₅ vs. control, ghrelin, Na₂S₂O₅ + ghrelin.

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