Chronic cholesterol treatment increased the cholesterol content in medial prefrontal cortex but not hippocampus. (a) Schemes and timeline of experimental schedules, Total cholesterol content in (b) medial prefrontal cortex, (c) hippocampus and (d) serum. Data are expressed as mean ± SEM (n = 8 per group). Differences between control and CMS were assessed using two-way ANOVA followed Tukey’s post hoc test. ***P < 0.001 compared with control rats. ##P < 0.01 compared with cholesterol-treated chronic mild stress rats.