Effects of Clostridium butyricum on antioxidant properties, meat quality and fatty acid composition of broiler birds

Table 2 Effect of C. butyricum on antioxidant indices in liver and serum of broilers

Item	Control	CB1	CB2	CB3	Antibiotic	Pooled SE	P-value
Liver^a
SOD (U/mg protein)	195c	212bc	240a	226ab	210bc	6	0.0002
GST (U/mg protein)	59.0	52.8	52.4	60.7	53.1	3.9	0.4546
GPX (U/mg protein)	8.49	9.45	9.19	8.67	9.26	0.36	0.2836
GSH (μmol/g protein)	10.09	9.39	10.32	9.39	9.36	0.50	0.5412
MDA (nmol/mg protein)	1.02a	0.88bc	0.82c	0.86bc	0.93ab	0.03	0.0014
Serum^a
SOD (U/ml)	118	110	128	113	107	9	0.5090
GST (U/ml)	22.9	22.7	22.4	21.5	19.8	0.8	0.0680
GPX (U)	785	797	786	867	890	46	0.4621
GSH (μmol/L)	29.9bc	32.5ab	38.0a	24.6 cd	22.1d	2.7	0.0016
MDA (nmol/ml)	2.36bc	2.37bc	2.24c	2.80ab	2.87a	0.16	0.0282

Means with different letters within the same row differ (P < 0.05).
Control = basal diet; CB1 = the basal diet supplemented with 2.5 × 10⁸ cfu of C. butyricum/kg; CB2 = the basal diet supplemented with 5 × 10⁸ cfu of C. butyricum/kg; CB3 = the basal diet supplemented with 1 × 10⁹ cfu of C. butyricum/kg; Antibiotic = the basal diet supplemented with 150 mg of aureomycin/kg.
SOD = superoxide dismutase; GST = glutathione S-transferase; GPX = glutathione peroxidase; GSH = glutathione; MDA = malondialdehyde.
^aEach value represents the mean of 8 cages with one chick per cage (n = 8 per treatment).

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